MGB Molecular Genetics and Molecular Biology Courses
Credit course for supervised participation in faculty research project. See page 40 for details.
DNA replication, DNA repair and mutation, recombination, transcription, RNA modification and processing, the genetic code and tRNA, translation, regulation of gene expression, development and differentiation, molecular evolution.
Laboratory experiments in genetics of selected organisms. Topics studied include Mendelian genetics, linkage and recombination, complementation, analysis of chromosome rearrangements, mutant selection and analysis in plants and bacteria, and genetic crosses with Drosophila, plants, bacteria and bacteriophages.
This course describes regulatory mechanisms controlling gene expression in prokaryotes and eukaryotes. The lectures are designed to promote discussion of various experimental approaches. Topics include: assembly of a transcription complex; initiation of transcription; role of sigma factors and transcription factors; role of regulators of transcription; regulation of SV40 transcription; lambda antitermination; antitermination in HIV-1.
This course presents and integrates molecular aspects of signal transduction and cell cycle regulation in eukaryotic cells from yeast to humans. Emphasis is on recent advances in growth factor receptor signalling, modular protein domains, and the recurrent role of protein phosphorylation and protein-protein interactions in cell regulation.
Laboratory experiments demonstrating basic and advanced molecular biological methods applied to molecular genetics. Methods include RNA and DNA purification, recombinant DNA methods, gel electrophoresis, PCR, immunoblots, and bioinformatic analysis.
Basic and advanced principles of genetic analysis applied to the study of two of the best-understood eukaryotic model organisms: the yeast Saccharomyces cerevisiae, and the nematode worm Caenorhabditis elegans. We emphasize the use of genetic approaches to address problems in cell biology and development, such as the regulation of cell fate. Much of the knowledge gained from these simple organisms has proven broadly applicable, and the same principles of developmental genetic analysis underlie efforts to understand the development of more complex organisms.
This is a companion course to MGB451H1. Advanced genetic principles and approaches, used in the study of fly and mouse development, are introduced. Emphasis is placed on developmental processes that are conserved among these major model organisms.
Structure, organization, expression and regulation of genes in the nucleus, and chloroplast of photosynthetic organisms; regulation of gene expression during plant development and in response to the environment.
Mutants of mammalian cells in culture, human cytogenetics and chromosome structure, human gene mapping, molecular genetic analysis and disease, oncongenes and cancer.
An opportunity for specialized individual research in molecular genetics and molecular biology by arrangement with the course coordinator.
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